Automated qPCR Pathogen Detection
KRAKEN™
Autonomous, field-deployable qPCR microbial detection and chemical testing system. Continuously samples in real-time, delivering results in as low as 60 minutes to ensure you always have quality data at your fingertips.
KRAKEN™ leverages advanced qPCR technology for rapid and accurate quantification without the need for enrichment, ensuring unparalleled sensitivity down to 1 copy. Customizable assays target a wide array of biological and chemical entities, with an open invitation for the development of new assays at no extra cost. Beyond detecting these targets, the platform's physical sensors measure pH, temperature, dissolved oxygen, conductivity, and redox potential, providing a comprehensive analysis. All data is securely managed and easily accessible via a cloud-based dashboard, enabling autonomous sampling and facilitating seamless trend analysis. The KRAKEN platform is not just a device; it's a groundbreaking solution that combines cutting-edge technology, adaptability, and precision to transform the landscape of monitoring and testing.
The KRAKEN™ System
The heart of Kraken Sense’s innovation lies in the KRAKEN™ device, a field-deployable marvel that utilizes advanced chemical and mechanical technology. By ingeniously bypassing the need for enrichment, it stands as a beacon of rapid real-time monitoring without compromising accuracy or quantification. Powered by gold-standard qPCR technology, it delivers quantification down to an astonishing 1 copy.
KRAKEN™ Reagents
Our custom-designed, novel assays redefine the landscape of testing. They allow us to bypass the conventional need for enrichment or cultivation while maintaining impeccable specificity and sensitivity. Complementing these assays is our specially formulated cleaning solution, safeguarding against cross-contamination and efficiently purifying the KRAKEN™ system from salts or biofilm-causing agents.
Secure Mobile Dashboard
Our cloud-based dashboard ensures safe and secure data sharing within your organization, fostering better traceability and facilitating effortless trend analysis. Everything you need is at your fingertips: detailed information on when and where samples were taken, all while enjoying the convenience of autonomous sampling. Simply schedule a run in the dashboard or initiate it remotely with the click of a button. With the KRAKEN™ system, you don’t need to be physically near the device to operate it. Additionally, the dashboard is equipped with intelligent analytics to off-load the heavy workload of post-processing data, further enhancing efficiency and insights.
Biological and Chemical Targets: Customization for Your Needs
At Kraken Sense, we understand that each industry and application demands precise detection. That's why our system is customizable to target a wide array of biological and chemical entities. Some of our readily available targets include E. coli, Salmonella, Legionella, Listeria monocytogenes, Vibrio, SARS-CoV-2, Monkeypox, opioids, domoic acid, crAssphage, Campylobacter, influenza, respiratory syncytial virus (RSV), and pepper mild mottle virus (PMMoV). Explore our complete range of available targets here.
But our commitment doesn’t end there. We're eager to expand our repertoire and develop new assays to meet your specific needs. Contact us with your target of interest, and our dedicated R&D team will develop an assay tailored for you at no additional cost.
Physical Sensors: Comprehensive Monitoring for Precision Analysis
In addition to detecting biological and chemical targets, the KRAKEN™ device boasts a suite of physical sensors ensuring a comprehensive analysis. It measures pH, temperature, conductivity, and turbidity, providing a holistic view of the sampled environment. What sets us apart is our adaptability; upon request, additional sensors can be seamlessly integrated into your customized device. We prioritize precision and flexibility, empowering you to gather a diverse range of critical data specific to your application or industry.
How KRAKEN™ works?
Remote Scheduling
Autonomous Sampling
Sample Quality Metrics¹
Cell Lysis²
DNA/RNA Concentration
qPCR Amplification
Calibration³
Automatic System Cleansing⁴
¹Sample Quality Metrics
Our system integrates physical sensors to ensure robust sample quality assessment, analyzing pH, temperature, dissolved oxygen, conductivity, and redox potential.
²Cell Lysis and Genetic Material Concentration
Cell lysis is a crucial step where microbial cells are gently opened to extract their genetic material. This step is fundamental as it allows us to access and concentrate the genetic essence necessary for precise analysis.
³Calibration allows adjustments for medium matrix fluctuations, ensuring consistent and reliable results.
⁴Automatic System Cleanse
The KRAKEN™ system features a specialized cleansing solution that effectively eradicates biofilms, maintaining system integrity and accuracy.
What is qPCR?
Quantitative Polymerase Chain Reaction (qPCR) amplification is a groundbreaking technique that rapidly and precisely multiplies segments of the extracted genetic material. This amplification process reveals the presence and quantity of specific microbial DNA or RNA, unlocking the identity and abundance of target pathogens or genetic markers.
In qPCR, small segments of DNA or RNA are multiplied through cycles of heating and cooling, allowing for exponential replication. By monitoring this amplification process in real-time, we achieve high-precision quantification, detecting even minute quantities of target genetic material.
The combination of cell lysis, concentration, and qPCR amplification forms the core of our microbial monitoring process. It's through these meticulous steps that we identify and quantify microbial entities, ensuring rapid, accurate, and reliable detection.
Features & Benefits
Real-time pathogen detection
With on-board sampling and data analysis, results are available in just 60 minutes without laboratory intervention.
Fully autonomous continuous monitoring
Samples are autonomously taken at any interval rate set by the operator, requiring no manual sample preparation.
Accurate multiplex detection system
Our devices monitor for multiple pathogens simultaneously, using controls to eliminate false positive and negative results.
Strain-specific quantitative results
Our state-of-the-art detection sensors are very sensitive to particular pathogenic species and strains without succumbing to background interference from other contaminants.
Secure cloud-based data storage
Live updates are sent to the web-based dashboard. Data is fully encrypted and securely stored in a cloud database for trend analysis.
Simple installation and maintenance
KRAKEN™ only requires power, WiFi/GSM connection, and access to a water line. Sensors are replaced every 66 tests for minimal maintenance.
We are very grateful to Kraken Sense for their ability to work with us, to customize the system to meet the needs of Toronto Pearson [Airport]; and I highly recommend Kraken Sense to any organization out there that is looking to have on-site pathogen testing available.
Success Story
Director of Safety, Security, & Health Greater Toronto Airport Authority
Dwayne Macintosh
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KRAKEN™ Product Brochure
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